Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles.
Neuron
; 38(2): 237-52, 2003 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-12718858
ABSTRACT
Recent studies indicate that active zones (AZs)-sites of neurotransmitter release-may be assembled from preassembled AZ precursor vesicles inserted into the presynaptic plasma membrane. Here we report that one putative AZ precursor vesicle of CNS synapses-the Piccolo-Bassoon transport vesicle (PTV)-carries a comprehensive set of AZ proteins genetically and functionally coupled to synaptic vesicle exocytosis. Time-lapse imaging reveals that PTVs are highly mobile, consistent with a role in intracellular transport. Quantitative analysis reveals that the Bassoon, Piccolo, and RIM content of individual PTVs is, on average, half of that of individual presynaptic boutons and shows that the synaptic content of these molecules can be quantitatively accounted for by incorporation of integer numbers (typically two to three) of PTVs into presynaptic membranes. These findings suggest that AZs are assembled from unitary amounts of AZ material carried on PTVs.
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Base de dados:
MEDLINE
Assunto principal:
Neuropeptídeos
/
Terminações Pré-Sinápticas
/
Proteínas de Ligação ao GTP
/
Vesículas Transportadoras
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Proteínas do Citoesqueleto
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article