[Methoxypolyethylene glycol-modified HLA-A2 antigen on lymphocytes].
Di Yi Jun Yi Da Xue Xue Bao
; 23(6): 557-60, 2003 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-12810374
ABSTRACT
OBJECTIVE:
To modify the HLA-A2 antigen on the lymphocytes with methoxypolyethylene glycol (mPEG) so as to block the specific binding site for antibody.METHOD:
Different types of mPEG (all with final concentration of 12 mmol/L) were used at different temperatures in PBS with varied pH values for the modification of the HLA-A2 antigen.RESULT:
The modification of the antigen was not obviously affected when it was carried out at 4 degrees Celsius or room temperature, but higher temperatures of 30 and 37 degrees Celsius significantly hampered the modification. Better antigen modification was observed with high-concentration mPEG in basic PBS, depending also on the type of mPEGs used for this purpose.CONCLUSION:
The specific HLA-A2 binding on the lymphocytes is completely blocked by benzotriazole carbonate-mPEG(mPEG-BTC), which is superior to N-hydroxysuccinimidyl ester of mPEG(mPEG-SPA). Maleimide-mPEG(mPEG-MAL) is incapable of blocking the HLA-A2 ligand-binding site with antibody.
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Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
Linfócitos
/
Antígeno HLA-A2
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article