Assessment of utility of meiosis-associated promoters of lily for induction of germinal ds transposition in transgenic rice.
Plant Cell Physiol
; 44(6): 637-42, 2003 Jun.
Article
em En
| MEDLINE
| ID: mdl-12826629
ABSTRACT
In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.
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Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Elementos de DNA Transponíveis
/
Regiões Promotoras Genéticas
/
Germinação
/
Lilium
/
Meiose
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article