[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2].
Zhonghua Zhong Liu Za Zhi
; 25(3): 220-4, 2003 May.
Article
em Zh
| MEDLINE
| ID: mdl-12839680
ABSTRACT
OBJECTIVE:
To investigate the apoptotic inducing effect of As(2)S(2) on K562 cells.METHODS:
The apoptotic inducing effect of As(2)S(2) on K562 cells was determined by flow cytometry, DNA fragmentation analysis and morphology observation. Expression of protein was determined by Western-blot. RT-PCR was used to evaluate changes in gene expression.RESULTS:
Apoptosis of K562 cells was induced by 48 - 72 h exposure to 5 micromol/L As(2)S(2). Apoptosis was induced in (34.4 +/- 3.3)% treated cells by 72 h exposure to 3 micro mol/L As(2)S(2), in (21.8 +/- 3.6)% treated cells by 48 h exposure to 5 micromol/L As(2)S(2) and in (46.0 +/- 5.2)% treated cells by 72 h exposure to As(2)S(2) at the same concentration. With 5 micromol/L As(2)S(2), the protein level of Bcr-Abl and JAK2 decreased, while bax expression was upregulated and c-myc was downregulated both in protein and mRNA level. The activity of caspase 3 in K562 cells was increased by As(2)S(2). As(2)S(2) also induced apoptosis of fresh mononuclear cells derived from chronic myelogenous leukemia (CML) patients.CONCLUSION:
As(2)S(2) can induce apoptosis of CML cells. The decline of Bcr-Abl may play an important role. The upregulation of bax, increase of the activity of caspase 3, downregulation of c-myc and decrease of JAK2 may also be involved in the mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Arsenicais
/
Sulfetos
/
Apoptose
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article