[Effect of T3 on the expression of transferrin receptor and ferritin in K562 cells and its possible mechanism].
Zhonghua Xue Ye Xue Za Zhi
; 24(4): 181-4, 2003 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-12864947
ABSTRACT
OBJECTIVE:
To explore the effect of T(3) on the expression of transferrin receptor (TfR) and ferritin (Fn) in K562 cells and its possible mechanism.METHODS:
Flow cytometry was used for the detection of TfR expression, radioimmunoassay for Fn expression, RNA/protein band shift assay for the binding activity of iron regulatory protein (IRP) and iron responsive elements (IRE), and RT-PCR for TfR and Fn mRNA levels.RESULTS:
Different concentration of T(3) significantly increased Fn expression of K562 cells, especially at 100 nmol/L and 200 nmol/L (p < 0.05). However, T(3) had no effect on TfR expression. T(3) decreased the binding activity between IRP and IRE, particularly at concentration of 50 nmol/L. Different concentration of T(3) increased Fn-H mRNA level at different time point while it had no effect on TfR mRNA level.CONCLUSION:
T(3) increased Fn expression of K562 cells through the possible mechanisms of either the post-transcriptional regulation or transcriptional modulation.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Tri-Iodotironina
/
Receptores da Transferrina
/
Ferritinas
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article