Angiotensin II modulates gene expression of adrenomedullin receptor components in rat cardiomyocytes.
Life Sci
; 73(13): 1629-35, 2003 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-12875895
ABSTRACT
Both adrenomedullin (AM) and angiotensin II (Ang II) are locally-acting hormones in the cardiac ventricles. Previously we reported that AM inhibits Ang II-induced hypertrophy of cultured rat neonatal cardiomyocytes. In this study, we examined whether Ang II affects the gene expression of the AM receptor components of calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein (RAMP) in rat cardiomyocytes. The mRNA levels of RAMP1 and RAMP3 were significantly elevated following 24-h treatment with Ang II without a change of those of RAMP2 and CRLR. AM increased the intracellular cAMP level and the cAMP accumulation by AM was significantly amplified by the 24-h preincubation with Ang II. The effects of Ang II on RAMP1 and RAMP3 expression were abolished by an Ang II type 1 (AT1) receptor antagonist, but not by an AT2 receptor antagonist. Thus, Ang II modulates gene expression of the AM receptor components via AT1 receptor, suggesting alteration of AM actions by Ang II in cultured rat cardiomyocytes.
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Base de dados:
MEDLINE
Assunto principal:
Angiotensina II
/
Expressão Gênica
/
Receptores de Peptídeos
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Receptores da Calcitonina
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Miócitos Cardíacos
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Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article