Expression of recombinant human ICOS and in vitro characterization of its bioactivity on B lymphocytes.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 35(7): 601-5, 2003 Jul.
Article
em En
| MEDLINE
| ID: mdl-12883628
ABSTRACT
Inducible costimulator (ICOS) is a novel costimulatory molecule expressed in activated T cell and has critical regulation effect on special immune response. In this study, the cDNA encoding human ICOS was cloned from activated tonsil cells via RT-PCR, and was expressed in E. coli on pET28 expression vector. The recombinant ICOS protein expressed from E. coli showed a molecular weight of 14 kD on SDS-polyacrylamide gel electrophoresis and was further confirmed by Western blot. In presence of IL-10, the purified rhICOS significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM), and enhanced the secretion of IgG from B cells.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Linfócitos B
/
Antígenos de Diferenciação de Linfócitos T
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article