Determination of proliferation index in neoplasms using different Ki-67 equivalent antibodies.
APMIS
; 111(5): 567-70, 2003 May.
Article
em En
| MEDLINE
| ID: mdl-12887508
ABSTRACT
Paraffin sections from 23 tumours were immunohistochemically stained with the following four Ki-67 equivalent antibodies monoclonal MIB-1 (DAKO), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (DAKO). Ki-67 labelling indices were determined by counting in exactly the same area in each case. MIB-1 showed the highest labelling index in 21 of the 23 cases, and the mean MIB-1 index was approximately 30% higher than that of the other antibodies. The differences between MM1, NCL-Ki-67p and Rah Ki-67 were small and non-significant. There was a positive correlation between each of the four antibodies. As these findings may be of importance when the Ki-67 labelling index is used as a criterion for tumour grading or for clinical prognostication, this necessitate identification of the antibody used in every case.
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Base de dados:
MEDLINE
Assunto principal:
Antígeno Ki-67
/
Neoplasias
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article