Myelin basic protein has multiple calmodulin-binding sites.
Biochem Biophys Res Commun
; 308(2): 313-9, 2003 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-12901870
ABSTRACT
Myelin basic protein (MBP) has been shown to bind calmodulin (CaM) in a specific Ca(2+)-dependent manner via a primary target sequence at its C-terminus [Protein Sci. 12 (2003) 1507]. Upon deimination of MBP, the nature of the interaction changed significantly, suggesting either a new binding site or different conformers with different affinities for CaM. In order to resolve this issue, we investigated here the CaM-binding properties of N- and C-terminal deletion mutants of MBP using Trp fluorescence spectroscopy and mass spectrometry. We conclude that there is an additional CaM-binding site on MBP in a central segment (we posit murine residues 82-93) that forms an amphipathic alpha-helix.
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Base de dados:
MEDLINE
Assunto principal:
Calmodulina
/
Proteína Básica da Mielina
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article