Cutting edge: H-2DM is responsible for the large differences in presentation among peptides selected by I-Ak during antigen processing.
J Immunol
; 171(5): 2183-6, 2003 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-12928360
ABSTRACT
We quantitated the amounts of peptides from hen egg-white lysozyme presented by I-A(k) molecules in APC lines. The large chemical gradient of presentation of the four hen egg-white lysozyme epitopes observed in cell lines expressing HLA-DM or H-2DM (referred to in this study as DM) was significantly diminished in the T2.A(k) line lacking DM. Differences in levels of presentation between wild-type and DM-deficient APC were observed for all four epitopes, but differences were most evident for the highest affinity epitope. As a result of these quantitative differences in display, presentation of all four epitopes to T cells was impaired in the line lacking DM. The binding affinity of the pool of naturally processed peptides from DM-expressing lines was higher than that from the DM-deficient line. Thus, using a direct biochemical approach in APC, we demonstrate that DM influences the selection of peptides bound to MHC class II by favoring high affinity peptides.
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Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Antígenos HLA-D
/
Antígenos de Histocompatibilidade Classe II
/
Apresentação de Antígeno
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article