FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers.
Mol Cell Probes
; 17(4): 165-9, 2003 Aug.
Article
em En
| MEDLINE
| ID: mdl-12944118
ABSTRACT
Locked Nucleic Acids (LNA) constitute a novel class of DNA analogues that have an exceptionally high affinity towards complementary DNA and RNA. Using human classical satellite-2 repeat sequence clusters as targets, we demonstrate that LNA/DNA mixmers oligonucleotides are excellent probes for FISH combining high binding affinity with short hybridization time and even with the ability to hybridize without prior thermal denaturation of the template.
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Base de dados:
MEDLINE
Assunto principal:
DNA Satélite
/
Sondas de DNA
/
Sondas de Oligonucleotídeos
/
Oligonucleotídeos Antissenso
/
Hibridização in Situ Fluorescente
Tipo de estudo:
Evaluation_studies
Limite:
Humans
/
Male
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article