Inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell proliferation.
World J Gastroenterol
; 9(9): 2117-20, 2003 Sep.
Article
em En
| MEDLINE
| ID: mdl-12970919
ABSTRACT
AIM:
To study the inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell line SMMC-7721 and to explore the mechanism of its effect.METHODS:
SMMC-7721 cells were divided into two groups, one treated with all-trans retinoic acid (ATRA) for 5 days and the other as a control group. Light microscope and electron microscope were used to observe the morphological changes. Telomerase activity was analyzed with silver-stained telomere repeated assay protocal (TRAP). Expression of Caspase-3 was demonstrated with western blot.RESULTS:
ATRA-treated cells showed differentiation features including small and pyknotic nuclei, densely stained chromatin and fewer microvilli. Besides, ATRA could inhibit the activity of telomerase, promote the expression of Caspase-3 and its activation.CONCLUSION:
Telomerase activity and Caspase-3 expression are changed in human hepatocellular carcinoma cell line SMMC-7721 treated with all-trans retinioc acid. The inhibition of telomerase activity and the activation of Caspase-3 may be the key steps through which ATRA inhibits the proliferation of SMMC-7721 cell line.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tretinoína
/
Carcinoma Hepatocelular
/
Neoplasias Hepáticas
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article