Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3.
Nat Genet
; 1(1): 34-9, 1992 Apr.
Article
em En
| MEDLINE
| ID: mdl-1301997
ABSTRACT
A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 19
/
DNA
/
Proteínas Proto-Oncogênicas c-fos
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Análise de Sequência de DNA
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article