Rescue of erythroid development in gene targeted GATA-1- mouse embryonic stem cells.
Nat Genet
; 1(2): 92-8, 1992 May.
Article
em En
| MEDLINE
| ID: mdl-1302015
ABSTRACT
Development of definitive (fetal liver-derived) red cells is blocked by a targeted mutation in the gene encoding the transcription factor GATA-1. We used in vitro differentiation of GATA-1- mouse embryonic stem (ES) cells to reveal a requirement for GATA-1 during primitive (yolk sac-derived) erythropoiesis and to establish a rescue assay. We show that the block to development includes primitive, as well as definitive, erythroid cells and is complete at the level of globin RNA expression; that the introduction of a normal GATA-1 gene restores developmental potential both in vivo and in vitro; and that efficient rescue is dependent on a putative autoregulatory GATA-motif in the distal promoter. Use of in vitro differentiated ES cells bridges a gap between conventional approaches to gene function in cell lines and analysis of loss of function mutations in the whole animal.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Fatores de Transcrição
/
Proteínas de Ligação a DNA
/
Eritropoese
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article