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Characterization of a yeast artificial chromosome contig spanning the Huntington's disease gene candidate region.
Bates, G P; Valdes, J; Hummerich, H; Baxendale, S; Le Paslier, D L; Monaco, A P; Tagle, D; MacDonald, M E; Altherr, M; Ross, M.
Afiliação
  • Bates GP; Genome Analysis Laboratory, Imperial Cancer Research Fund, London, UK.
Nat Genet ; 1(3): 180-7, 1992 Jun.
Article em En | MEDLINE | ID: mdl-1303232
The Huntington's disease (HD) gene has been localized by recombination events to a region covering 2.2 megabases (Mb) DNA within chromosome 4p16.3. We have screened three yeast artificial chromosome (YAC) libraries in order to isolate and characterize 44 YAC clones mapping to this region. Approximately 50% of the YACs were chimaeric. Unstable YACs were identified across the whole region, but were particularly prevalent around the D4S183 and D4S43 loci. The YACs have been assembled into a contig extending from D4S126 to D4S98 covering roughly 2 Mb DNA, except for a gap of about 250 kilobases (kb). The establishment of a YAC contig which spans the region most likely to contain the HD mutation is an essential step in the isolation of the HD gene.
Assuntos
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Base de dados: MEDLINE Assunto principal: Doença de Huntington Limite: Humans Idioma: En Ano de publicação: 1992 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Doença de Huntington Limite: Humans Idioma: En Ano de publicação: 1992 Tipo de documento: Article