A quantitative assay of retrograde transported HSV in the trigeminal ganglion.
Invest Ophthalmol Vis Sci
; 33(6): 1934-9, 1992 May.
Article
em En
| MEDLINE
| ID: mdl-1316334
ABSTRACT
The relationship between the dose of Herpes simplex virus type 1 (HSV) inoculated in the cornea and the amount of actively replicating virus recovered from mouse trigeminal ganglion cells 5 d after corneal scratch and inoculation was investigated with a tissue culture plaque assay. A dose response curve of productive viral replication was obtained. The estimated dose of HSV that produces half-maximal recovery of virus within the ganglion was 9.15 x 10(3) plaque forming units per eye, and the maximal amount of HSV recovered was 1.34 x 10(4) pfu per ganglion. This definition of infectivity as a function of dose will be useful for studying the effects of potential inhibitors of the binding, uptake, and transport of HSV by productively or latently infected trigeminal neurons.
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Base de dados:
MEDLINE
Assunto principal:
Gânglio Trigeminal
/
Ceratite Herpética
/
Herpes Simples
Limite:
Animals
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article