Direct expression of active spinach glycolate oxidase in Escherichia coli.
Biochim Biophys Acta
; 1132(1): 11-6, 1992 Aug 17.
Article
em En
| MEDLINE
| ID: mdl-1324737
ABSTRACT
Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expressed in E. coli has identical physico-chemical features to the wild-type enzyme, but is expressed at a level approx. 15-fold higher than in the yeast system.
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Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Oxirredutases do Álcool
/
Escherichia coli
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article