Localization of an immunoinhibitory epitope of the cysteine proteinase, cathepsin L.
Immunol Invest
; 21(6): 495-506, 1992 Oct.
Article
em En
| MEDLINE
| ID: mdl-1385322
ABSTRACT
Antibodies, raised in chickens (IgY) and rabbits (IgG) against the lysosomal proteinase cathepsin L, targeted the enzyme in an ELISA and Western blot. In contrast to the rabbit IgG, the chicken IgY was immunoinhibitory towards cathepsin L. An epitope that elicits immunoinhibitory antibodies has been localized to an active site-associated peptide sequence. The corresponding free peptide, coated down in an ELISA, is recognised by the chicken IgY, but not the rabbit IgG. This peptide was able to inhibit the immunoinhibition of cathepsin L by chicken anti-cathepsin L IgY, suggesting its complete or partial identity with an immunogenic epitope for chickens in whole cathepsin L.
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Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Cisteína Endopeptidases
/
Catepsinas
Limite:
Animals
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article