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Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein.
Wahlberg, J M; Bron, R; Wilschut, J; Garoff, H.
Afiliação
  • Wahlberg JM; Department of Molecular Biology, Karolinska Institute, Novum, Huddinge, Sweden.
J Virol ; 66(12): 7309-18, 1992 Dec.
Article em En | MEDLINE | ID: mdl-1433520
ABSTRACT
Infection of cells with enveloped viruses is accomplished through membrane fusion. The binding and fusion processes are mediated by the spike proteins in the envelope of the virus particle and usually involve a series of conformational changes in these proteins. We have studied the low-pH-mediated fusion process of the alphavirus Semliki Forest virus (SFV). The spike protein of SFV is composed of three copies of the protein heterodimer E2E1. This structure is resistant to solubilization in mild detergents such as Nonidet P-40 (NP40). We have recently shown that the spike structure is reorganized during virus entry into acidic endosomes (J. M. Wahlberg and H. Garoff, J. Cell Biol. 116339-348, 1992). The original NP40-resistant heterodimer is dissociated, and the E1 subunits form new NP40-resistant protein oligomers. Here, we show that the new oligomer is represented by an E1 trimer. From studies that use an in vitro assay for fusion of SFV with liposomes, we show that the E1 trimer is efficiently expressed during virus-mediated membrane fusion. Time course studies show that both E1 trimer formation and fusion are fast processes, occurring in seconds. It was also possible to inhibit virus binding and fusion with a monoclonal antibody directed toward the trimeric E1. These results give support for a model in which the E1 trimeric structure is involved in the SFV-mediated fusion reaction.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus da Floresta de Semliki / Proteínas do Envelope Viral / Proteínas Virais de Fusão / Lipossomos / Fusão de Membrana Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 1992 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vírus da Floresta de Semliki / Proteínas do Envelope Viral / Proteínas Virais de Fusão / Lipossomos / Fusão de Membrana Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 1992 Tipo de documento: Article