c-fos mRNA constitutive expression by mature human megakaryocytes.
Oncogene
; 7(11): 2341-4, 1992 Nov.
Article
em En
| MEDLINE
| ID: mdl-1437157
ABSTRACT
By in situ hybridization with a c-fos probe, we have shown that human bone marrow megakaryocytes cultured in the presence of 20% aplastic anemia plasma constitutively express c-fos mRNA. At day 0, megakaryocytes are mostly immature and only 3% of them are labeled. The number of labeled cells reached 23% after 12 days of culture. Interleukin 3 (IL-3) and IL-6 added together at day 10 further increased this number to 31% 2 days later. Mature labeled megakaryocytes were more numerous and more strongly labeled than immature ones. These results suggest that c-fos could play a role in megakaryocytic terminal differentiation, either in the polyploidization or in the thrombopoietic function unique to these cells.
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Megacariócitos
/
Genes fos
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article