Your browser doesn't support javascript.
loading
Identification of PDGFR as a receptor for AAV-5 transduction.
Di Pasquale, Giovanni; Davidson, Beverly L; Stein, Colleen S; Martins, Inês; Scudiero, Dominic; Monks, Anne; Chiorini, John A.
Afiliação
  • Di Pasquale G; Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nat Med ; 9(10): 1306-12, 2003 Oct.
Article em En | MEDLINE | ID: mdl-14502277
Understanding the process of vector transduction has important implications for the application and optimal use of a vector system for human gene therapy. Recent studies with vectors based on adeno-associated virus type 5 (AAV-5) have shown utility of this vector system in the lung, central nervous system, muscle and eye. To understand the natural tropism of this virus and to identify proteins necessary for AAV-5 transduction, we characterized 43 cell lines as permissive or nonpermissive for AAV-5 transduction and compared the gene expression profiles derived from cDNA microarray analyses of those cell lines. A statistically significant correlation was observed between expression of the platelet-derived growth factor receptor (PDGFR-alpha-polypeptide) and AAV-5 transduction. Subsequent experiments confirmed the role of PDGFR-alpha and PDGFR-beta as receptors for AAV-5. The tropism of AAV-5 in vivo also correlated with the expression pattern of PDGFR-alpha.
Assuntos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Transdução Genética / Receptores do Fator de Crescimento Derivado de Plaquetas / Dependovirus Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2003 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Transdução Genética / Receptores do Fator de Crescimento Derivado de Plaquetas / Dependovirus Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2003 Tipo de documento: Article