Subset-specific, uniform activation among V gamma 6/V delta 1+ gamma delta T cells elicited by inflammation.
J Leukoc Biol
; 75(1): 68-75, 2004 Jan.
Article
em En
| MEDLINE
| ID: mdl-14525969
ABSTRACT
The V gamma 6/V delta 1(+) cells, the second murine gamma delta T cell subset to arise in the thymus, express a nearly invariant T cell receptor (TCR), colonize select tissues, and expand preferentially in other tissues during inflammation. These cells are thought to help in regulating the inflammatory response. Until now, V gamma 6/V delta 1(+) cells have only been detectable indirectly, by expression of V gamma 6-encoding mRNA. Here, we report that 17D1, a monoclonal antibody, which detects the related epidermis-associated V gamma 5/V delta 1(+) TCR, will also bind the V gamma 6/V delta 1(+) cells if their TCR is first complexed to an anti-C delta antibody. Features of this special condition for recognition suggest the possibility that an alternate structure exists for the V gamma 6/V delta 1 TCR, which is stabilized upon binding to the anti-C delta antibody. Using the 17D1 antibody as means to track this gamma delta T cell subset by flow cytometry, we discovered that the response of V gamma 6/V delta 1(+) cells during inflammation often far exceeds that of other subsets and that the responding V gamma 6/V delta 1(+) cells display a strikingly uniform activation/memory phenotype compared with other gamma delta T cell subsets.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Subpopulações de Linfócitos T
/
Receptores de Antígenos de Linfócitos T gama-delta
/
Inflamação
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article