Media for hybridoma growth and monoclonal antibody production.

ABSTRACT

For the economical production of monoclonal antibodies (MAbs), the cell-culture medium must be optimized for three different phases growth of the hybridomas, MAb productivity of the hybridomas, and MAb purification or downstream processing. Medium improvements are necessary to meet these requirements for large-scale MAb production. Information bearing on this issue is being addressed in two research areas, cell biology and biochemical engineering, and is reviewed in this article.