Capillary electrophoretic separation of heparin oligosaccharides under conditions amenable to mass spectrometric detection.
J Chromatogr A
; 1014(1-2): 225-33, 2003 Oct 03.
Article
em En
| MEDLINE
| ID: mdl-14558628
ABSTRACT
A capillary electrophoresis method for the separation of high-molecular-mass heparin oligosaccharides compatible with mass spectral detection was developed. Structurally defined heparin oligosaccharides ranging in size from tetrasaccharide to tetradecasaccharide were used to optimize the conditions. Applying normal and reversed polarity modes, these oligosaccharides were separated by CE under various conditions. Ammonium hydrogencarbonate (30 mM at pH 8.50) used as the running electrolyte system gave good separation efficiency and resolution in the normal polarity mode. Application of this method to the separation of complicated heparin oligosaccharide mixtures required the addition of electrolyte additives. Ammonium hydrogencarbonate (30 mM), containing triethylamine (10 mM), was useful for the separation of complex oligosaccharide mixtures. Run-to-run and day-to-day precision and limits of detection were established for these separations.
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Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Espectrometria de Massas
/
Heparina
/
Eletroforese Capilar
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article