A novel technique with enhanced detection and quantitation of HPV-16 E1- and E2-mediated DNA replication.
Virology
; 315(1): 103-9, 2003 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-14592763
ABSTRACT
Transient DNA replication assays to detect papillomavirus E1/E2-mediated DNA replication have depended upon Southern blotting. This technique is hazardous (radioactive), labour intensive, semiquantitative, and physically limited in the number of samples that can be processed at any one time. We have overcome these problems by developing a real-time PCR protocol for the detection of E1/E2-mediated transient DNA replication. The results demonstrate detection of replication at levels not seen using Southern blotting demonstrating enhanced sensitivity. This technique is also, by definition, highly quantitative. Therefore, the real-time PCR technique is the optimal method for the detection of E1/E2-mediated DNA replication.
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Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Proteínas Oncogênicas Virais
/
Reação em Cadeia da Polimerase
/
Proteínas de Ligação a DNA
/
Replicação do DNA
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article