RGS16 inhibits signalling through the G alpha 13-Rho axis.
Nat Cell Biol
; 5(12): 1095-103, 2003 Dec.
Article
em En
| MEDLINE
| ID: mdl-14634662
ABSTRACT
G alpha 13 stimulates the guanine nucleotide exchange factors (GEFs) for Rho, such as p115Rho-GEF. Activated Rho induces numerous cellular responses, including actin polymerization, serum response element (SRE)-dependent gene transcription and transformation. p115Rho-GEF contains a Regulator of G protein Signalling domain (RGS box) that confers GTPase activating protein (GAP) activity towards G alpha 12 and G alpha 13 (ref. 3). In contrast, classical RGS proteins (such as RGS16 and RGS4) exhibit RGS domain-dependent GAP activity on G alpha i and G alpha q, but not G alpha 12 or G alpha 13 (ref 4). Here, we show that RGS16 inhibits G alpha 13-mediated, RhoA-dependent reversal of stellation and SRE activation. The RGS16 amino terminus binds G alpha 13 directly, resulting in translocation of G alpha 13 to detergent-resistant membranes (DRMs) and reduced p115Rho-GEF binding. RGS4 does not bind G alpha 13 or attenuate G alpha 13-dependent responses, and neither RGS16 nor RGS4 affects G alpha 12-mediated signalling. These results elucidate a new mechanism whereby a classical RGS protein regulates G alpha 13-mediated signal transduction independently of the RGS box.
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Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Proteínas
/
Proteínas rho de Ligação ao GTP
/
Proteínas RGS
/
Subunidades alfa G12-G13 de Proteínas de Ligação ao GTP
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article