Crystallization of RusA Holliday junction resolvase from Escherichia coli.

Muranova, Tatyana A; Sedelnikova, Svetlana E; Leonard, Philip M; Pasquo, Alessandra; Bolt, Edward L; Lloyd, Robert G; Rafferty, John B

Muranova, Tatyana A; Sedelnikova, Svetlana E; Leonard, Philip M; Pasquo, Alessandra; Bolt, Edward L; Lloyd, Robert G; Rafferty, John B.

Afiliação

Muranova TA; Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, England.

Acta Crystallogr D Biol Crystallogr ; 59(Pt 12): 2262-4, 2003 Dec.

Article em En | MEDLINE | ID: mdl-14646089

ABSTRACT

ABSTRACT

Crystals of the Escherichia coli Holliday junction resolvase RusA have been obtained using the hanging-drop method and characterized. The crystals have a primitive monoclinic form and belong to space group P2(1). The V(M) value suggests the presence of two copies of the monomer in the asymmetric unit. A full three-wavelength MAD data collection on a selenomethionine-incorporated form has been undertaken and structure determination is under way using data collected to 2.1 A resolution.

Assuntos

Proteínas de Escherichia coli/química; Escherichia coli/enzimologia; Resolvases de Junção Holliday/química; Cristalização/métodos; Cristalografia por Raios X/métodos; Proteínas de Escherichia coli/genética; Resolvases de Junção Holliday/genética; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Selenometionina/química

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Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli / Resolvases de Junção Holliday / Escherichia coli Idioma: En Ano de publicação: 2003 Tipo de documento: Article

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