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Cannabinoid CB1 receptor and serotonin 3 receptor subunit A (5-HT3A) are co-expressed in GABA neurons in the rat telencephalon.
Morales, Marisela; Wang, Shwun-De; Diaz-Ruiz, Oscar; Jho, Diana Hyun-Jin.
Afiliação
  • Morales M; National Institute on Drug Abuse, Intramural Research Program, Cellular Neurophysiology, Baltimore, Maryland 21224, USA. mmorales@intra.nida.nih.gov
J Comp Neurol ; 468(2): 205-16, 2004 Jan 06.
Article em En | MEDLINE | ID: mdl-14648680
ABSTRACT
Among all described serotonin (5-HT) receptors in mammals, the type three (5-HT3) is the only ligand-gated ion channel receptor for serotonin. By using double in situ hybridization histochemistry, we found co-expression of the functional 5-HT3A subunit of the 5-HT3 receptor and the central CB1 cannabinoid receptor in neurons of the rat telencephalon. Double-labeled 5-HT3A/CB1 neurons were found in the anterior olfactory nucleus, superficial and deep layers of the cortex, hippocampal formation (hippocampus, dentate gyrus, subiculum, and entorhinal cortex) and amygdala. Analysis of the proportion of neurons co-expressing 5-HT3A and CB1 receptors in the cortex and amygdala showed that, depending on the brain region, 37-53% of all neurons expressing the 5-HT3A subunit also expressed CB1 transcripts; 16-72% of the total population of neurons expressing CB1 mRNA co-expressed the 5-HT3A subunit. By using a combination of double in situ hybridization and immunohistochemistry, we demonstrated that 5-HT3A/CB1-expressing neurons contained the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). These results imply that in distinct regions of the telencephalon, GABA neurons that react to cannabinoids may also be responsive to serotonin through 5-HT3 receptors. Cellular coexistence of 5-HT3A and CB1 transcripts in interneurons of the cortex, hippocampal formation, and amygdala suggest possible interactions between the cannabinoid and serotonergic systems at the level of GABA neurotransmission in brain areas involved in cognition, memory, and emotion.
Assuntos
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Base de dados: MEDLINE Assunto principal: Telencéfalo / Receptor CB1 de Canabinoide / Receptores 5-HT3 de Serotonina / Ácido gama-Aminobutírico / Neurônios Limite: Animals Idioma: En Ano de publicação: 2004 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Telencéfalo / Receptor CB1 de Canabinoide / Receptores 5-HT3 de Serotonina / Ácido gama-Aminobutírico / Neurônios Limite: Animals Idioma: En Ano de publicação: 2004 Tipo de documento: Article