FcepsilonRI-dependent gene expression in human mast cells is differentially controlled by T helper type 2 cytokines.
J Allergy Clin Immunol
; 112(6): 1119-26, 2003 Dec.
Article
em En
| MEDLINE
| ID: mdl-14657870
ABSTRACT
BACKGROUND:
Mast cells (MCs) proliferate in response to T(H)2 cytokines and express genes de novo after activation. Limited information is available concerning the interplay between these events.OBJECTIVE:
We explored the potential for T(H)2 cytokines to alter activation-dependent gene expression by MCs.METHODS:
Cord blood-derived human (h)MCs maintained in stem cell factor (SCF) alone were compared with replicates treated with IL-4, IL-5, or IL-9, respectively, for their patterns of FcepsilonRI-dependent gene induction using microarray technology.RESULTS:
Activation of SCF-treated hMCs upregulated their expression of roughly 140 transcripts at 2 hours, including genes involved in cell cycle progression and arrest. Each cytokine substantially modified this profile; approximately 800 inducible genes apiece were controlled by IL-5 or IL-9, whereas 169 inducible genes were controlled by IL-4. IL-4 favored the induction of cytokines and of genes associated with cell growth arrest (GADD34, GAS-1, CIDE-A, INK4D, and BAX) and completely abolished the enhanced proliferation observed in the other 3 groups after activation. Conversely, IL-5 priming induced preferential upregulation of genes involved in cell proliferation and did not abolish thymidine incorporation.CONCLUSIONS:
T(H)2 cytokines differentially modulate gene induction in hMCs after FcepsilonRI cross-linkage. IL-4 uniquely controls cytokine gene expression by hMCs and might also limit their activation-driven proliferation.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina E
/
Receptores Fc
/
Regulação da Expressão Gênica
/
Citocinas
/
Células Th2
/
Mastócitos
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article