Distinct domains of the limbic system-associated membrane protein (LAMP) mediate discrete effects on neurite outgrowth.
Mol Cell Neurosci
; 24(3): 725-40, 2003 Nov.
Article
em En
| MEDLINE
| ID: mdl-14664821
ABSTRACT
The limbic system-associated membrane protein (LAMP) is a glycosylphosphatidylinositol-anchored glycoprotein with three immunoglobulin (Ig) domains that can either enhance or inhibit neurite outgrowth depending upon the neuronal population examined. In the present study, we investigate the domains responsible for these activities. Domain deletion revealed that the N-terminal IgI domain is necessary and sufficient for the neurite-promoting activity observed in hippocampal neurons. In contrast, inhibition of neurite outgrowth in SCG neurons, which is mediated by heterophilic interactions, requires full-length LAMP, although selective inhibition of the second Ig domain, but not the first or third domains, prevented the inhibitory effect. This indicates that the IgII domain of LAMP harbors the neurite-inhibiting activity, but only in the context of the full-length configuration. Covasphere-binding analyses demonstrate IgI/IgI interactions, but no interaction between IgII and any other domain, consistent with the biological activities that each domain mediates. The data suggest that LAMP may serve as a bifunctional guidance molecule, with distinct structural domains contributing to the promotion and inhibition of neurite outgrowth.
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Base de dados:
MEDLINE
Assunto principal:
Moléculas de Adesão Celular Neuronais
/
Diferenciação Celular
/
Neuritos
/
Sistema Nervoso
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article