Transgenic RNAi reveals essential function for CTCF in H19 gene imprinting.
Science
; 303(5655): 238-40, 2004 Jan 09.
Article
em En
| MEDLINE
| ID: mdl-14716017
ABSTRACT
The imprinted regulation of H19 and Insulin-like growth factor 2 expression involves binding of the vertebrate insulator protein, CCCTC binding factor (CTCF), to the maternally hypomethylated differentially methylated domain (DMD). How this hypomethylated state is maintained during oogenesis and the role of CTCF, if any, in this process are not understood. With the use of a transgenic RNA interference (RNAi)-based approach to generate oocytes with reduced amounts of CTCF protein, we found increased methylation of the H19 DMD and decreased developmental competence of CTCF-deficient oocytes. Our results suggest that CTCF protects the H19 DMD from de novo methylation during oocyte growth and is required for normal preimplantation development.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Proteínas Repressoras
/
Impressão Genômica
/
RNA não Traduzido
/
Interferência de RNA
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Proteínas de Ligação a DNA
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article