Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors.
Nat Med
; 10(2): 203-7, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14716306
ABSTRACT
Transport parameters determine the access of drugs to tumors. However, technical difficulties preclude the measurement of these parameters deep inside living tissues. To this end, we adapted and further optimized two-photon fluorescence correlation microscopy (TPFCM) for in vivo measurement of transport parameters in tumors. TPFCM extends the detectable range of diffusion coefficients in tumors by one order of magnitude, and reveals both a fast and a slow component of diffusion. The ratio of these two components depends on molecular size and can be altered in vivo with hyaluronidase and collagenase. These studies indicate that TPFCM is a promising tool to dissect the barriers to drug delivery in tumors.
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Base de dados:
MEDLINE
Assunto principal:
Microscopia de Fluorescência
/
Neoplasias
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article