[Preliminary study on the gene expression profiles of ependymomas with cDNA array].
Zhonghua Wai Ke Za Zhi
; 41(10): 770-2, 2003 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-14766053
ABSTRACT
OBJECTIVE:
To investigate the differential gene expression of ependymomas.METHODS:
Four fresh samples of ependymomas and 1 of normal brain tissue were collected during operation. The extracted total RNAs were converted as (32)P tagged cDNA probes, which were then hybridized with the Atlas Human Cancer Array, producing the array based hybridization maps following the protocol provided with the kit. A set of special software was applied to the analysis and RT-PCR was performed to test the result.RESULT:
In comparison with the normal brain tissue, there were 31 upregulated gene and 1 downregulated gene in ependymomas, most of which were firstly found to be differentially expressed in this kind of tumor.CONCLUSION:
The discrepancy of gene expression profiles between ependymomas and normal brain tissues is highly put through and effectively detected with cDNA array, which provides new information for the further research on the molecular mechanisms of this lesion.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Encefálicas
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Ependimoma
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article