Simultaneous suppression of epidermal growth factor receptor and c-erbB-2 reverses aneuploidy and malignant phenotype of a human ovarian carcinoma cell line.
Cancer Res
; 64(3): 789-94, 2004 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-14871800
ABSTRACT
Coexpression of epidermal growth factor receptor (EGFR) and c-erbB-2 in 47-68% of ovarian cancer cells indicate their strong association with tumor formation. We examined the effects of simultaneous antisense- or immunosuppression of EGFR and c-erbB-2 expression on the invasive phenotype, aneuploidy, and genotype of cultured human ovarian carcinoma cells (NIHOVCAR-8). We report here that suppression of both EGFR and c-erbB-2 results in regression of aneuploidy and genomic imbalances in NIHOVCAR-8 cells, restores a more normal phenotype, and results in a more normal gene expression profile. Combined with cytogenetic analysis, our data demonstrate that the regression of aneuploidy is due to the selective apoptosis of double antisense transfected cells with highly abnormal karyotype.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Receptor ErbB-2
/
Receptores ErbB
/
Aneuploidia
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article