NF-kappaB binds to a polymorphic repressor element in the MMP-3 promoter.
Biochem Biophys Res Commun
; 316(1): 182-8, 2004 Mar 26.
Article
em En
| MEDLINE
| ID: mdl-15003528
ABSTRACT
A 5T/6T polymorphic site in the matrix metalloproteinase-3 (MMP-3) promoter has been identified as a repressor element involved in inhibiting induction of MMP-3 transcription by interleukin 1; and the 6T allele has been associated with decreased expression of MMP-3 as compared to the 5T allele. Zinc-binding protein-89 (ZBP-89) was cloned from a yeast one-hybrid assay via its ability to interact with this site, but when the protein was over-expressed, it resulted in activation of the MMP-3 promoter rather than repression. Here we show that in nuclear extracts isolated from human gingival fibroblasts stimulated with IL-1, this site is bound by p50 and p65 components of NF-kappaB in addition to ZBP-89, and that recombinant p50 binds preferentially to the 6T binding site. These results are consistent with a role for NF-kappaB in limiting the cytokine induced expression of MMP-3.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
NF-kappa B
/
Regiões Promotoras Genéticas
/
Metaloproteinase 3 da Matriz
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article