Detection of transfusion transmitted virus DNA by real-time PCR.
J Clin Virol
; 29(4): 277-81, 2004 Apr.
Article
em En
| MEDLINE
| ID: mdl-15018856
ABSTRACT
BACKGROUND:
Little is known about the pathogenic role and the endemic situation of transfusion transmitted virus (TTV).OBJECTIVES:
In this study, a molecular assay for detection of TTV based on automated nucleic acid extraction and real-time PCR was developed and evaluated. The new assay includes an internal control. STUDYDESIGN:
After optimization of the molecular assay, 103 clinical samples were studied retrospectively. All sera had been tested for anti-HCV and anti-HIV-1 antibodies earlier.RESULTS:
The amplification efficiency was found to be 102%. When clinical specimens were tested, 79 of 103 serum samples were found to be positive for TTV. There was no significant difference between various groups of patients. The internal control was detected in all negative and weak positive samples.CONCLUSIONS:
This molecular assay proved to be suitable for routine detection of TTV in clinical samples. Moreover, a relative statement on the TTV serum load can be done.
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Torque teno virus
/
Infecções por Vírus de DNA
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article