LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress.
Mol Cells
; 17(1): 125-31, 2004 Feb 29.
Article
em En
| MEDLINE
| ID: mdl-15055538
ABSTRACT
The CD53 antigen is a member of the tetraspanin membrane protein family that is expressed in the lymphoid-myeloid lineage. Its biological role remains unknown. Using microarrays, we identified CD53 as one of the principal genes up-regulated by exposure of macrophages to LPS. Northern blot analysis confirmed the induction of CD53 in RAW264.7 macrophages treated with LPS or SNAP (a nitric oxide donor). Cells stably transfected with sense CD53 cDNA had increased levels of intracellular GSH and lower levels of peroxide, and were more resistant to H2O2 and to UVB irradiation. Cells harboring antisense CD53 had the opposite properties. We propose that the induction of CD53 is a major mechanism by which macrophages protect themselves against LPS-induced oxidative stress and UVB irradiation.
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Base de dados:
MEDLINE
Assunto principal:
Penicilamina
/
Antígenos de Diferenciação de Linfócitos T
/
Antígenos CD
/
Lipopolissacarídeos
/
Estresse Oxidativo
/
Macrófagos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article