Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p.
Nat Cell Biol
; 6(5): 414-9, 2004 May.
Article
em En
| MEDLINE
| ID: mdl-15077114
ABSTRACT
Myristoylation of ARF family GTPases is required for their association with Golgi and endosomal membranes, where they regulate protein sorting and the lipid composition of these organelles. The Golgi-localized ARF-like GTPase Arl3p/ARP lacks a myristoylation signal, indicating that its targeting mechanism is distinct from myristoylated ARFs. We demonstrate that acetylation of the N-terminal methionine of Arl3p requires the NatC N(alpha)-acetyltransferase and that this modification is required for its Golgi localization. Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p.
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Base de dados:
MEDLINE
Assunto principal:
Acetiltransferases
/
Fatores de Ribosilação do ADP
/
Proteínas de Saccharomyces cerevisiae
/
Complexo de Golgi
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article