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Structural and conformational aspects of the anticoagulant and anti-thrombotic activity of heparin and dermatan sulfate.
Casu, Benito; Guerrini, Marco; Torri, Giangiacomo.
Afiliação
  • Casu B; Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy. casu@ronzoni.it
Curr Pharm Des ; 10(9): 939-49, 2004.
Article em En | MEDLINE | ID: mdl-15078125
ABSTRACT
Heparin and other iduronic acid-containing glycosaminoglycans (GAG) such as dermatan sulfate exert their anticoagulant properties primarily by accelerating the rate of inhibition of the natural protease inhibitors antithrombin III (AT, which inhibits both factor Xa and thrombin) and heparin cofactor II (HCII, which selectively inhibits thrombin). Although AT and HCII are structural homologs, only heparin binds to AT, and HCII has different binding sites for heparin and dermatan sulfate. Whereas the binding site of heparin for AT is a unique pentasaccharide sequence contained in only about one third of the chains of this GAG, HCII-binding sequences of heparin and dermatan sulfate are less specific and contained in practically all the GAG chains. Protein binding and associated biological activities of heparin and dermatan sulfate are modulated by the "plasticity" of their iduronic acid residues due to the availability of up to three equienergetic conformation among which the protein selects the one favouring the most stable complex. Glycol-splitting of nonsulfated uronic acid residues, a device for generating flexible joints along the GAG chains, has different effects on different binding domains. Whereas it inactivates the binding site for AT causing a drop of the anticoagulant activity, it enhances the HCII-associated activity of both heparin and dermatan sulfate.
Assuntos
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Base de dados: MEDLINE Assunto principal: Heparina / Dermatan Sulfato / Fibrinolíticos / Anticoagulantes Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2004 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Heparina / Dermatan Sulfato / Fibrinolíticos / Anticoagulantes Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2004 Tipo de documento: Article