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[(3)H]Adenosine uptake in brainstem membranes of CD-1 mice lacking the adenosine A(2a) receptor.
Snell, Broughton J; Day, Anne; Ledent, Catherine; Lawrence, Andrew J.
Afiliação
  • Snell BJ; Department of Pharmacology, Faculty of Medicine, Monash University, Clayton, Victoria, Australia.
Life Sci ; 75(2): 225-35, 2004 May 28.
Article em En | MEDLINE | ID: mdl-15120574
Previous studies in our laboratory have demonstrated a decrease in [(3)H]nitrobenzylthioinosine binding sites in the brainstem of adenosine A(2a) receptor knockout mice, particularly in the brain nuclei involved in central control of cardiovascular function [Brain Research 877 (2000) 160]. The present study aimed to correlate this decrease, shown using autoradiography, with a functional change using a previously described method of [(3)H]adenosine uptake in a membrane preparation from the brainstem of wildtype CD - 1 and homozygous mutant mice lacking the adenosine A(2a) receptor. A statistically significant decrease was shown in the mean V(MAX) value obtained from homozygous mutant preparations (4.7 +/- 1.3 fmol/mg protein/20 s, P < 0.05, n = 4) compared to that obtained from wildtype controls (51.6 +/- 4.2 fmol/mg protein/20 s, n = 4). Competition studies using nucleoside uptake inhibitors showed a statistically significant increase in the log IC(50) values for dipyridamole (Wildtype: -4.3 +/- 0.2, Homozygous mutant: -8.3 +/- 0.4, n=5, P < 0.05) and dilazep (Wildtype: -3.9 +/- 0.8, Homozygous mutant: -8.3 +/- 0.8, n=5, P < 0.05) in the preparations using homozygous mutant tissue. The present study, in conjunction with the results of previous studies [Brain Research 877 (2000) 160], indicates that components of purinergic neurotransmission system have apparently adjusted in compensation for the lack of the A(2a) receptor.
Assuntos
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Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Tronco Encefálico / Adenosina / Receptores A2 de Adenosina Limite: Animals Idioma: En Ano de publicação: 2004 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Tronco Encefálico / Adenosina / Receptores A2 de Adenosina Limite: Animals Idioma: En Ano de publicação: 2004 Tipo de documento: Article