Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase.
Carbohydr Res
; 339(10): 1727-37, 2004 Jul 12.
Article
em En
| MEDLINE
| ID: mdl-15220082
ABSTRACT
Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of alpha-amylases, and are useful in rapidly screening for novel alpha-amylase inhibitors and for subsequent kinetic characterisation.
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Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
Alfa-Amilases
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article