Photosensitization with protoporphyrin IX inhibits attachment of cancer cells to a substratum.
Biochem Biophys Res Commun
; 322(2): 452-7, 2004 Sep 17.
Article
em En
| MEDLINE
| ID: mdl-15325251
ABSTRACT
Effects of photodynamic therapy (PDT) on adhesion of human adenocarcinoma cells of the line WiDr to a plastic substratum were investigated. Protoporphyrin IX induced by 5-aminolevulinic acid (ALA) was used as a photosensitizer. Light exposure inhibited attachment of suspended cells to a substratum. The adhesion was most strongly pronounced for light exposures around 200 mJ/cm(2) causing cell death. However, sub-lethal exposures (42 mJ/cm(2), 97% survival) inhibited cell adhesion as well. Sub-lethal ALA-PDT increased the intracellular space in dense colonies of WiDr cells. This was attributed to formation of lamellipodia between the cells and to increased numbers of focal contacts containing alpha(V)beta(3) integrin in some of the cells. The E-cadherin distribution was not changed by the treatment. Complex processes, including changes in cellular shape and reorganization of the cytoskeleton, are suggested to participate in the observed ALA-PDT effect on the cell adhesion.
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Base de dados:
MEDLINE
Assunto principal:
Protoporfirinas
/
Fármacos Fotossensibilizantes
/
Neoplasias
Limite:
Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article