Effects of transdifferentiation and EGF on claudin isoform expression in alveolar epithelial cells.
J Appl Physiol (1985)
; 98(1): 322-8, 2005 Jan.
Article
em En
| MEDLINE
| ID: mdl-15361518
ABSTRACT
Rat alveolar epithelial type II cells grown on polycarbonate filters form high-resistance monolayers and concurrently acquire many phenotypic properties of type I cells. Treatment with EGF has previously been shown to increase transepithelial resistance across alveolar epithelial cell (AEC) monolayers. We investigated changes in claudin expression in primary cultured AEC during transdifferentiation to the type I cell-like phenotype (days 0, 1, and 8), and on day 5 in culture +/- EGF (10 ng/ml) from day 0 or day 4. Claudins 4 and 7 were increased, whereas claudins 3 and 5 were decreased, on later compared with earlier days in culture. Exposure to EGF led to increases in claudins 4 and 7 and decreases in claudins 3 and 5. Claudin 1 was only faintly detectable in freshly isolated type II cells and remained unchanged over time in culture and after exposure to EGF. These results suggest that increases in transepithelial resistance accompanying AEC transdifferentiation and/or EGF exposure are mediated, at least in part, by changes in the pattern of expression of specific claudin isoforms.
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Base de dados:
MEDLINE
Assunto principal:
Alvéolos Pulmonares
/
Junções Íntimas
/
Fator de Crescimento Epidérmico
/
Células Epiteliais
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article