The crucial role of Campylobacter jejuni genes in anti-ganglioside antibody induction in Guillain-Barre syndrome.
J Clin Invest
; 114(11): 1659-65, 2004 Dec.
Article
em En
| MEDLINE
| ID: mdl-15578098
ABSTRACT
Molecular mimicry of Campylobacter jejuni lipo-oligosaccharides (LOS) with gangliosides in nervous tissue is considered to induce cross-reactive antibodies that lead to Guillain-Barre syndrome (GBS), an acute polyneuropathy. To determine whether specific bacterial genes are crucial for the biosynthesis of ganglioside-like structures and the induction of anti-ganglioside antibodies, we characterized the C. jejuni LOS biosynthesis gene locus in GBS-associated and control strains. We demonstrated that specific types of the LOS biosynthesis gene locus are associated with GBS and with the expression of ganglioside-mimicking structures. Campylobacter knockout mutants of 2 potential GBS marker genes, both involved in LOS sialylation, expressed truncated LOS structures without sialic acid, showed reduced reactivity with GBS patient serum, and failed to induce an anti-ganglioside antibody response in mice. We demonstrate, for the first time, to our knowledge, that specific bacterial genes are crucial for the induction of anti-ganglioside antibodies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
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Lipopolissacarídeos
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Campylobacter jejuni
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Mimetismo Molecular
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Síndrome de Guillain-Barré
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Gangliosídeos
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article