Role of beta2-integrins for homing and neovascularization capacity of endothelial progenitor cells.
J Exp Med
; 201(1): 63-72, 2005 Jan 03.
Article
em En
| MEDLINE
| ID: mdl-15623573
ABSTRACT
The mechanisms of homing of endothelial progenitor cells (EPCs) to sites of ischemia are unclear. Here, we demonstrate that ex vivo-expanded EPCs as well as murine hematopoietic Sca-1+/Lin- progenitor cells express beta2-integrins, which mediate the adhesion of EPCs to endothelial cell monolayers and their chemokine-induced transendothelial migration in vitro. In a murine model of hind limb ischemia, Sca-1+/Lin- hematopoietic progenitor cells from beta2-integrin-deficient mice are less capable of homing to sites of ischemia and of improving neovascularization. Preactivation of the beta2-integrins expressed on EPCs by activating antibodies augments the EPC-induced neovascularization in vivo. These results provide evidence for a novel function of beta2-integrins in postnatal vasculogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
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Movimento Celular
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Antígenos CD18
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Neovascularização Fisiológica
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Células Endoteliais
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Isquemia
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article