[Generation and identification of rice T-DNA insertional mutant lines].
Yi Chuan Xue Bao
; 31(12): 1388-94, 2004 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-15633645
ABSTRACT
By using the matured embryos of Japonica rice variety Zhonghua No. 11 as explants, rice transformation was performed by Agrobacterium-mediated co-cultivation method, resulting in 1489 independent transgenic rice plants that carry a T-DNA insertion. Genomic DNA gel-blot and PCR analyses showed that 69.8% of the total lines contain the inserted T-DNA. The flanking sequence of T-DNA in transgenic rice plants was analyzed using Tail-PCR. In addition, we have evaluated 1066 T1 transgenic lines on heading days, plant height and panicles per hill, and found different types of mutants from a number of lines.
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Base de dados:
MEDLINE
Assunto principal:
Oryza
/
DNA Bacteriano
/
Mutagênese Insercional
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article