Simultaneous estimation of a Y-specific fragment, an X-specific fragment and sex determination of forensic studies in real-time PCR.
Forensic Sci Int
; 146 Suppl: S165-6, 2004 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-15639567
ABSTRACT
The human sex test in forensic multiplexes is based on the amelogenin gene on both the X and Y chromosomes commonly used in sex genotyping. In this work sex determination across the quantification of nuclear DNA was achieved by real-time PCR of a segment of the X-Y homologous amelogenin (AMG) gene that allowed the simultaneous estimation of a Y- and an X-specific fragment. The AMG real-time PCR design has been used to quantify a set of forensic casework samples.
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Impressões Digitais de DNA
/
Processos de Determinação Sexual
/
Proteínas do Esmalte Dentário
Tipo de estudo:
Observational_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article