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P-glycoprotein expression affects the intracellular concentration and antiviral activity of the protease inhibitor saquinavir in a T cell line.
Maffeo, A; Bellomi, F; Solimeo, I; Bambacioni, F; Scagnolari, C; De Pisa, F; Dupuis, M L; Cianfriglia, M; Antonelli, G; Turriziani, O.
Afiliação
  • Maffeo A; Virology Section, Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy.
New Microbiol ; 27(2 Suppl 1): 119-26, 2004 Apr.
Article em En | MEDLINE | ID: mdl-15646074
ABSTRACT
A number of ATP-binding cassette proteins, which function as cellular efflux pumps, are known to be expressed on the membranes of human cells, including CD4-positive lymphocytes. It has also been shown recently that most anti-HIV protease inhibitors (PIs) are first-rate substrates of one of these membrane transporters, P-glycoprotein (Pgp). These findings raise the question of whether Pgp expression could influence HIV replication and/or affect the action of PIs. To gain new insight into this, initially unexpected, phenomenon, a study was undertaken with the aims of investigating whether treatment with saquinavir (SQV) induces Pgp expression in primary or transformed human T cell lines and, primarily, establishing whether Pgp expression could modify both the uptake of SQV and its antiviral action. Pgp expression, mainly measured by reverse transcription-PCR, was found to be variably detectable in healthy individuals, and short or prolonged SQV treatment was unable to induce or increase the expression of Pgp in a lymphoblastoid cell line or in primary lymphocytes derived from these individuals. However, further experiments, performed in a cell line with high Pgp expression (CEM(VBL100) cells) and its parental cell line (CEM cells), demonstrated that over-expression of Pgp reduces the uptake of SQV This result is consistent with the finding that CEM(VBL100) cells are less sensitive to the antiviral activity of SQV, the ID50 value (100 microM) being significantly higher than the corresponding value in parental CEM cells (4 microM).
Assuntos
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Base de dados: MEDLINE Assunto principal: Linfócitos T / HIV / Inibidores da Protease de HIV / Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Saquinavir Limite: Humans Idioma: En Ano de publicação: 2004 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Linfócitos T / HIV / Inibidores da Protease de HIV / Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Saquinavir Limite: Humans Idioma: En Ano de publicação: 2004 Tipo de documento: Article