Interactions of proteoliposomes from serogroup B Neisseria meningitidis with bone marrow-derived dendritic cells and macrophages: adjuvant effects and antigen delivery.
Vaccine
; 23(10): 1312-21, 2005 Jan 26.
Article
em En
| MEDLINE
| ID: mdl-15652674
ABSTRACT
Exposure to proteoliposomes from serogroup B Neisseria meningitidis (PL) induced up-regulation of MHC-II, MHC-I, CD40, CD80 and CD86 expression on the surface of murine bone marrow-derived dendritic cells (DC). CD40, CD80 and CD86 were up-regulated on bone marrow-derived macrophages (MPhi) upon stimulation with PL. Both DC and MPhi released TNFalpha, but only DC produced IL12(p70) in response to PL. A small increase in the expression of MHC-II, CD40 and CD86, as well as production of IL12(p70), was observed on the cell surface of DC, but not MPhi from LPS-non-responder C3H/HeJ after exposure to PL. DC, but not MPhi, incubated with PL containing ovalbumin (PL-OVA) presented OVA-specific peptides to CD4+ and CD8+ OVA-specific T-cell hybridomas. These data clearly indicate that PL exert an immunomodulatory effect on DC and MPhi, with some contribution of non-LPS components besides the main role of LPS. The work also shows the potential of PL as a general system to deliver antigens to DC for presentation to CD4+ and CD8+ T-cells.
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Base de dados:
MEDLINE
Assunto principal:
Proteolipídeos
/
Células Dendríticas
/
Células da Medula Óssea
/
Adjuvantes Imunológicos
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Vacinas Meningocócicas
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Neisseria meningitidis Sorogrupo B
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Macrófagos
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article