A computational and experimental approach to validating annotations and gene predictions in the Drosophila melanogaster genome.
Proc Natl Acad Sci U S A
; 102(5): 1566-71, 2005 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-15668397
ABSTRACT
Five years after the completion of the sequence of the Drosophila melanogaster genome, the number of protein-coding genes it contains remains a matter of debate; the number of computational gene predictions greatly exceeds the number of validated gene annotations. We have assembled a collection of >10,000 gene predictions that do not overlap existing gene annotations and have developed a process for their validation that allows us to efficiently prioritize and experimentally validate predictions from various sources by sequencing RT-PCR products to confirm gene structures. Our data provide experimental evidence for 122 protein-coding genes. Our analyses suggest that the entire collection of predictions contains only approximately 700 additional protein-coding genes. Although we cannot rule out the discovery of genes with unusual features that make them refractory to existing methods, our results suggest that the D. melanogaster genome contains approximately 14,000 protein-coding genes.
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Base de dados:
MEDLINE
Assunto principal:
Genoma
/
Proteínas de Drosophila
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Drosophila melanogaster
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article